A conflict can be rooted in the main plot, it can be rooted in a relationship between characters, or it can be both. Here are a few strategies for creating a new conflict in a scene.
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Personal Relationships 105-02
Social Relationships
Most relationships include a social aspect, but a true social relationship is rooted in coming together to share an experience. Social relationships are based on a common interest or shared experience. A social relationship can be casual, two strangers meeting at an event, or they can be intentional, two friends who specifically gather to meet each other.
Immediate Tension 104-04
Within a scene each character has a goal and obstacles preventing them from achieving that goal. This means every scene contains at least one conflict, if every character is collaborating or competing to achieve the same goal.
Creating Tension 104-03
Tension is created whenever a conflict prevents a character from achieving a goal. This can be a force of nature, another character, or an internal struggle, such as the character debating what to do. Tension also helps to create a sense of pacing, the rate at which scenes progress. Generally an audience will perceive a low tension scene as longer than a high tension scene.